Lesson eLearning

Which of the following correctly lists the three main steps of a Polymerase Chain Reaction (PCR) in the order they occur?

easy MCQ 1 mark Class 12 · Biology · Biotechnology and Its Applications
  1. Denaturation → Extension → Annealing
  2. Denaturation → Annealing → Extension
  3. Annealing → Denaturation → Extension
  4. Extension → Denaturation → Annealing
Answer
Denaturation → Annealing → Extension

The correct answer is Denaturation → Annealing → Extension — Arrange them in the order they occur during each cycle.

Step-by-step solution

  1. Identify the three stages of PCR as taught in the chapter: denaturation, annealing, and extension.
  2. Recall that the temperature cycles start with high temperature to separate DNA strands (denaturation).
  3. Next, the temperature is lowered to allow primers to bind (annealing).
  4. Finally, DNA polymerase extends the primers at an optimal temperature (extension).
  5. Arrange them in the order they occur during each cycle.
Common mistake: Confusing the order of annealing and extension temperatures

Concept tested: PCR cycle steps

Published · How this solution was prepared and checked

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