Which promoter would most likely give the highest level of transgene expression in both tomato fruit and the pseudostem?
Answer
CaMV 35S promoter
The correct answer is CaMV 35S promoter — Thus, CaMV 35S is the best choice for strong expression in both fruit and pseudostem.
Step-by-step solution
- Recall that CaMV 35S is a constitutive viral promoter active in many plant tissues, including fruit and vegetative parts.
- Note that the E8 promoter is fruit‑specific and has minimal activity in pseudostem.
- Understand that AtUBQ10 and rice actin promoters are also constitutive but generally show lower activity in tomato fruit.
- Thus, CaMV 35S is the best choice for strong expression in both fruit and pseudostem.
Common mistake: Choosing a fruit‑specific promoter for vegetative tissues
Concept tested: Promoter selection for tissue‑specific expression
Published · How this solution was prepared and checked
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