Lesson eLearning

Which promoter would most likely give the highest level of transgene expression in both tomato fruit and the pseudostem?

hard MCQ 4 marks Class 12 · Biology · Biotechnology and Its Applications
  1. CaMV 35S promoter
  2. E8 fruit‑specific promoter
  3. AtUBQ10 promoter
  4. Rice actin promoter
Answer
CaMV 35S promoter

The correct answer is CaMV 35S promoter — Thus, CaMV 35S is the best choice for strong expression in both fruit and pseudostem.

Step-by-step solution

  1. Recall that CaMV 35S is a constitutive viral promoter active in many plant tissues, including fruit and vegetative parts.
  2. Note that the E8 promoter is fruit‑specific and has minimal activity in pseudostem.
  3. Understand that AtUBQ10 and rice actin promoters are also constitutive but generally show lower activity in tomato fruit.
  4. Thus, CaMV 35S is the best choice for strong expression in both fruit and pseudostem.
Common mistake: Choosing a fruit‑specific promoter for vegetative tissues

Concept tested: Promoter selection for tissue‑specific expression

Published · How this solution was prepared and checked

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