Define gene cloning. Mention one commonly used cloning vector and state its key feature.
Answer
Gene cloning is the process of making multiple copies of a specific DNA segment by inserting it into a vector; a commonly used vector is pUC19, which has a high copy number and ampicillin resistance.
Step-by-step solution
- State that gene cloning involves isolating a DNA fragment of interest and inserting it into a DNA carrier (vector).
- Explain that the recombinant vector is introduced into a host cell where it is replicated, producing many copies of the fragment.
- Identify pUC19 as a widely used cloning vector.
- Highlight that pUC19 contains a high‑copy‑number origin of replication and an ampicillin‑resistance gene for easy selection.
- Combine the definition and vector description into the final answer.
Common mistake: Confusing gene cloning with bacterial transformation, which is the method of introducing the vector into cells
Concept tested: Definition and example of gene cloning
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