Lesson eLearning

Which of the following restriction enzymes generates blunt ends when it cuts DNA?

easy MCQ 1 mark Class 12 · Biology · Biotechnology Principles and Processes
  1. EcoRI
  2. HindIII
  3. SmaI
  4. BamHI
Answer
SmaI

The correct answer is SmaI — Select the enzyme that yields blunt ends.

Step-by-step solution

  1. Identify the type of ends produced by each enzyme.
  2. EcoRI cuts at GAATTC producing 5' overhangs (sticky ends).
  3. HindIII cuts at AAGCTT also producing 5' overhangs.
  4. BamHI cuts at GGATCC producing 5' overhangs.
  5. SmaI cuts at CCC and leaves no overhang, i.e., blunt ends.
  6. Select the enzyme that yields blunt ends.
Common mistake: Confusing enzymes that produce sticky ends with those that produce blunt ends.

Concept tested: Restriction enzyme cleavage patterns

Published · How this solution was prepared and checked

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